The opened processivity clamp slides into view
نویسندگان
چکیده
منابع مشابه
Structure and Mechanism of the DNA Polymerase Processivity Clamp Loader
The replication of a genome is a fundamental biological process that every organism must perform in order to transmit its genetic makeup to its progeny. We have been studying the molecular machines that are used to rapidly and accurately replicate the DNA genome of bacteria. In addition to the polymerase (the actual copying machine), organisms have a set of assemblies that enable the polymerase...
متن کاملCrystal Structure of the Processivity Clamp Loader Gamma (γ) Complex of E. coli DNA Polymerase III
ing it by 360Њ about eight times each second. To avoid the tangle that would otherwise result, the polymerase can release DNA, holding onto the fork via attachment to . This would allow the leading strand DNA to swivel Summary back into position within the  clamp (Hingorani and O'Donnell, 2000). The ␥ complex, an AAAϩ ATPase, is the bacterial ho-molog of eukaryotic replication factor C (RFC) ...
متن کاملExchange between Escherichia coli polymerases II and III on a processivity clamp
Escherichia coli has three DNA polymerases implicated in the bypass of DNA damage, a process called translesion synthesis (TLS) that alleviates replication stalling. Although these polymerases are specialized for different DNA lesions, it is unclear if they interact differently with the replication machinery. Of the three, DNA polymerase (Pol) II remains the most enigmatic. Here we report a sta...
متن کاملPolymerase exchange on single DNA molecules reveals processivity clamp control of translesion synthesis.
Translesion synthesis (TLS) by Y-family DNA polymerases alleviates replication stalling at DNA damage. Ring-shaped processivity clamps play a critical but ill-defined role in mediating exchange between Y-family and replicative polymerases during TLS. By reconstituting TLS at the single-molecule level, we show that the Escherichia coli β clamp can simultaneously bind the replicative polymerase (...
متن کاملAn artificial processivity clamp made with streptavidin facilitates oriented attachment of polymerase–DNA complexes to surfaces
Single molecule analysis of individual enzymes can require oriented immobilization of the subject molecules on a detection surface. As part of a technology development project for single molecule DNA sequencing, we faced the multiple challenges of immobilizing both a DNA polymerase and its DNA template together in an active, stable complex capable of highly processive DNA synthesis on a nonstic...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2005
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.0507120102